TG Antibody Cocktail / Thyroglobulin orb248559 from Biorbyt

Description

Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites, detected at ~300kDa in western blot. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for thyroglobulin antibody even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently thyroglobulin negative. Adenocarcinomas of nonthyroid origin do not react with this antibody cocktail. It is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the origin of the tumor. Thyroglobulin + calcitonin antibody, can identify medullary carcinomas of the thyroid. Furthermore, thyroglobulin + TTF1 antibody can be a reliable marker to differentiate between primary thyroid and lung neoplasms

Product Specs
ItemTG Antibody Cocktail / Thyroglobulin
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb248559
Quantity20 µg, 100 µg
ApplicationsIHC-P
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman, Mouse, Rat
TargetTG
IsotypeMouse IgG1, kappa
Clone2H11 + 6E1
HostMouse
ImmunogenHuman thyroid follicular cells were used as the immunogen for this Thyroglobulin antibody
NCBI Full Gene Namethyroglobulin
NCBI Gene AliasesAITD3, TGN
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Thyroglobulin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

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